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Comparative analysis of colorimetric staining in skin using open‐source software
Author(s) -
Billings Paul C.,
Sanzari Jenine K.,
Kennedy Ann R.,
Cengel Keith A.,
Seykora John T.
Publication year - 2015
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12594
Subject(s) - staining , immunohistochemistry , open source , software , positive staining , computer science , colorimetry , pathology , chemistry , medicine , computer vision , programming language
Colorimetric staining techniques such as immunohistochemistry ( IHC ), immunofluorescence ( IF ) and histochemistry ( HC ) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open‐source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation‐induced tanning response of Yucatan mini‐pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC / IF / HC samples.