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Hair follicle targeting, penetration enhancement and Langerhans cell activation make cyanoacrylate skin surface stripping a promising delivery technique for transcutaneous immunization with large molecules and particle‐based vaccines
Author(s) -
Vogt Annika,
Hadam Sabrina,
Deckert Iliane,
Schmidt Julia,
Stroux Andrea,
Afraz Zahra,
Rancan Fiorenza,
Lademann Jürgen,
Combadiere Behazine,
BlumePeytavi Ulrike
Publication year - 2015
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12589
Subject(s) - hair follicle , cyanoacrylate , penetration (warfare) , langerhans cell , immunization , intense pulsed light , medicine , immunology , nanotechnology , materials science , dermatology , antigen , adhesive , layer (electronics) , operations research , engineering
Abstract Transcutaneous immunization ( TCI ) requires targeting of a maximum number of skin antigen‐presenting cells as non‐invasive as possible on small skin areas. In two clinical trials, we introduced cyanoacrylate skin surface stripping ( CSSS ) as a safe method for TCI . Here, using ex vivo human skin, we demonstrate that one CSSS procedure removed only 30% of stratum corneum, but significantly increased the penetration of 200 nm polystyrene particles deep into vellus and intermediate hair follicles from where they could not been retrieved by conventional tape stripping. Two subsequent CSSS had no striking additional effect. CSSS increased particle penetration in superficial stratum corneum and induced Langerhans cell activation. Formulation in amphiphilic ointment or massage did not substantially influences the interfollicular penetration profiles. Hair follicle ( HF ) targeting by CSSS could become a highly effective tool for TCI when combined with carrier‐based delivery and is gaining new attention as our understanding on the HF immune system increases.