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Characterization of the novel Sezary lymphoma cell line BKP 1
Author(s) -
Boudjarane John,
Essaydi Arnaud,
Farnault Laure,
Popovici Cornel,
LafagePochitaloff Marina,
Beaufils Nathalie,
BerdaHaddad Yaël,
Lacroix Romaric,
NicolinoBrunet Corinne,
Le Treut Thérèse,
Zattara Hélène,
Gabert Jean,
KahnPerlès Brigitte,
Costello Régis
Publication year - 2015
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12567
Subject(s) - mycosis fungoides , comparative genomic hybridization , biology , immunophenotyping , cd8 , cd3 , lymphoma , t cell , t cell receptor , flow cytometry , cancer research , immune system , gene , microbiology and biotechnology , genetics , immunology , chromosome
Cutaneous T‐cell lymphomas (CTCL) are a heterogeneous group of lymphomas primarily involving the skin. The most common types are mycosis fungoides (MF) and Sezary Syndrome (SS). We report a novel long‐term fast‐growing SS line termed BKP1 that was characterized by flow cytometry (FC), conventional and molecular cytogenetic [FISH/multi‐FISH together with array comparative genomic hybridization ( aCGH )]. FC immunophenotype of the BKP1 is CD2+CD5+CD3+CD4+CD8−CD7−CD25−CD26−CD30−CD158k+. The TCR γ characterization of BKP1 by PCR identified a clonal rearrangement. The conventional cytogenetic and Multi‐FISH analysis showed complex chromosomal rearrangements. aCGH analysis highlighted the loss of genes involved in cell cycle control, in immune response ( HLA , complement complex) and DNA damage repair mechanisms. The BKP1 is another lymphoma cell line thoroughly characterized that can be a valuable tool for both basic and applied research such as identification of deregulated genes and/or pathways and screening for new antilymphoma drugs.