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Activation of autophagic pathways is related to growth inhibition and senescence in cutaneous squamous cell carcinoma
Author(s) -
Choi So Ra,
Chung Bo Young,
Kim Seong Who,
Kim Chang Deok,
Yun Woo Jin,
Lee Mi Woo,
Choi Jee Ho,
Chang Sung Eun
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12515
Subject(s) - autophagy , senescence , atf3 , apoptosis , cancer research , cell growth , programmed cell death , cell , biology , microbiology and biotechnology , cell culture , gene expression , biochemistry , genetics , promoter , gene
Cutaneous squamous cell carcinoma ( SCC ) is a very common resectable cancer; however, cutaneous SCC is highly resistant to chemotherapy if metastasis develops. Activating transcription factor 3 ( ATF 3) has been suggested as a marker of advanced or metastatic cutaneous SCC . Autophagy is one of the most important mechanisms in cancer biology and commonly induced by in vitro serum starvation. To investigate the role of autophagy activation in cutaneous SCC , we activated autophagic pathways by serum starvation in SCC 13 and ATF 3‐overexpressing SCC 13 ( ATF 3‐ SCC 13) cell lines. ATF 3‐ SCC 13 cells demonstrated high proliferative capacity and low p53 and autophagy levels in comparison with control SCC 13 cells under basal conditions. Intriguingly, autophagic stimulation via serum starvation resulted in growth inhibition and senescence in both cells, while ATF 3‐ SCC 13 cells further demonstrated growth inhibition and senescence. Apoptosis was not significantly induced by autophagy activation. Taken together, autophagy activation may be a promising antitumor approach for advanced cutaneous SCC .