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Perilipin 3 modulates specific lipogenic pathways in SZ 95 sebocytes
Author(s) -
Camera Emanuela,
Dahlhoff Maik,
Ludovici Matteo,
Zouboulis Christos C.,
Schneider Marlon R.
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12507
Subject(s) - perilipin , chemistry , microbiology and biotechnology , biochemistry , medicine , endocrinology , food science , biology , lipolysis , adipose tissue
Lipid droplets ( LD ) are dynamic organelles that manage cellular lipid synthesis, storage and retrieval. Although LD ‐associated proteins, including the perilipin family ( PLIN 1– PLIN 5), are essential for these functions, they have been poorly characterized in sebocytes. Here, we employed si RNA s to downregulate PLIN 3 in SZ 95 sebaceous gland cells and evaluated the consequences in lipid accumulation by nile red staining and mass spectrometry. Nile red staining revealed that si RNA ‐mediated downregulation of PLIN 3 significantly impaired linoleic acid‐induced lipid accumulation in SZ 95 sebocytes. Mass spectrometry revealed that PLIN 3 was implicated in the metabolism of linoleic acid, a lipid source used in the build‐up of triglycerides, among other acyl lipids. Furthermore, the expression of key enzymes of sebaceous lipogenesis was altered in PLIN 3‐deficient sebocytes, consistent with the changes observed in the neutral lipid abundance, suggesting that PLIN 3 functions are intertwined with the lipogenic pathways implicated in sebaceous lipogenesis, such as desaturation and triglyceride synthesis.