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Human dermal fibroblast migration induced by fibronectin in autocrine and paracrine manners
Author(s) -
Li Xiaoguang,
Qian Hua,
Ono Fumitake,
Tsuchisaka Atsunari,
Krol Rafal P.,
Ohara Koji,
Hayakawa Taihei,
Matsueda Satoko,
Sasada Tetsuro,
Ohata Chika,
Furumura Minao,
Hamada Takahiro,
Hashimoto Takashi
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12447
Subject(s) - autocrine signalling , paracrine signalling , hacat , fibronectin , cell migration , dermal fibroblast , microbiology and biotechnology , integrin , keratinocyte , fibroblast , chemistry , cell culture , biology , cell , extracellular matrix , biochemistry , receptor , genetics
Although fibronectin ( FN ) is known as a chemoattractant for human dermal fibroblasts ( HDF s), it is unclear whether HDF migration is stimulated by FN produced by HDF s (autocrine manner) or by keratinocytes (paracrine manner). In this study, we investigated HDF migration by Boyden chamber assay using conditioned media from HDF s and HaCaT cells (keratinocyte cell line). Immunoblotting and enzyme‐linked immunosorbent assay revealed that FN existed in both conditioned media. Boyden chamber assay showed both conditioned media stimulated HDF migration, which was inhibited by anti‐ FN antibody. Antibodies to both integrin β 1and β 3 subunits inhibited HDF migration induced by HDF ‐conditioned medium almost completely and that by HaCaT cell‐conditioned medium with 50–60%. These results suggested that HDF migration was stimulated by FN in both autocrine and paracrine manners. However, the mechanisms of HDF migration by FN , particularly the role of integrin β 1 and β 3 subunits, were slightly different between autocrine and paracrine manners.