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Role of E ts‐1 in fibronectin‐derived heparin‐binding domain polypeptides alleviating melanoma cell invasiveness and chemoresistance
Author(s) -
Tang Nanhong,
Wang Xiaoqian,
Huang Tao,
Wu Yong,
Chen Yuanzhong
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12444
Subject(s) - fibronectin , melanoma , heparin , cancer research , chemistry , metastasis , integrin , cell adhesion , recombinant dna , epirubicin , adhesion , microbiology and biotechnology , cell , biology , medicine , biochemistry , chemotherapy , cancer , organic chemistry , gene , cyclophosphamide
In this study, we observed that rh FNHN 29 and rh FNHC 36, two recombinant heparin‐binding domain polypeptides of fibronectin, suppressed adhesion and invasion of B 16 F 10 and A 375 melanoma cells mediated by integrin α v and α 2 in a dose‐dependent manner. Combined with low‐concentration epirubicin ( EPI ), rh FNHN 29 or rh FNHC 36 exhibited a synergistic inhibition on the viability and metastasis of B 16 F 10 cells. Moreover, in the presence of high‐concentration rh FNHN 29 or rh FNHC 36, the E ts‐1 activity and the expression of p‐ FAK , p‐ E rk1/2 and E ts‐1 were notably downregulated in B 16 F 10 cells. Ets‐1 is one of the central regulatory links for rh FNHN 29 and rh FNHC 36 to suppress the adhesion and invasion of melanoma cells. Combining rh FNHN 29 or rh FNHC 36 with EPI may be a good way to alleviate invasiveness or chemoresistance in melanoma.