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Comparison of structure and organization of cutaneous lipids in a reconstructed skin model and human skin: spectroscopic imaging and chromatographic profiling
Author(s) -
Tfayli Ali,
Bonnier Franck,
Farhane Zeineb,
Libong Danielle,
Byrne Hugh J.,
BailletGuffroy Arlette
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12423
Subject(s) - stratum corneum , human skin , ceramide , barrier function , chemistry , chromatography , biochemistry , pathology , biology , medicine , apoptosis , genetics , microbiology and biotechnology
The use of animals for scientific research is increasingly restricted by legislation, increasing the demand for human skin models. These constructs present comparable bulk lipid content to human skin. However, their permeability is significantly higher, limiting their applicability as models of barrier function, although the molecular origins of this reduced barrier function remain unclear. This study analyses the stratum corneum ( SC ) of one such commercially available reconstructed skin model ( RSM ) compared with human SC by spectroscopic imaging and chromatographic profiling. Total lipid composition was compared by chromatographic analysis ( HPLC ). Raman spectroscopy was used to evaluate the conformational order, lateral packing and distribution of lipids in the surface and skin/ RSM sections. Although HPLC indicates that all SC lipid classes are present, significant differences are observed in ceramide profiles. Raman imaging demonstrated that the RSM lipids are distributed in a non‐continuous matrix, providing a better understanding of the limited barrier function.