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Gluteal and abdominal subcutaneous adipose tissue depots as stroma cell source: gluteal cells display increased adipogenic and osteogenic differentiation potentials
Author(s) -
Iwen Karl Alexander,
Priewe AnnaChristin,
Winnefeld Marc,
Rose Christian,
Siemers Frank,
Rohwedel Jürgen,
Cakiroglu Figen,
Lehnert Hendrik,
Schepky Andreas,
Klein Johannes,
Kramer Jan
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12406
Subject(s) - adipose tissue , adipogenesis , depot , stem cell , medicine , pathology , biology , microbiology and biotechnology , archaeology , history
Human adipose‐derived stroma cells ( ADSC s) have successfully been employed in explorative therapeutic studies. Current evidence suggests that ADSC s are unevenly distributed in subcutaneous adipose tissue; therefore, the anatomical origin of ADSC s may influence clinical outcomes. This study was designed to investigate proliferation and differentiation capacities of ADSC s from the gluteal and abdominal depot of 8 females. All had normal BMI (22.01 ± 0.39 kg/m 2 ) and waist circumference (81.13 ± 2.33 cm). Examination by physicians and analysis of 31 laboratory parameters did not reveal possibly confounding medical disorders. Gluteal and abdominal adipose tissue was sampled by en bloc resection on day 7 (±1) after the last menses. Histological examination did not reveal significant depot‐specific differences. As assessed by BrdU assay, proliferation of cells from both depots was similar after 24 h and analysis of 15 cell surface markers by flow cytometry identified the isolated cells as ADSC s, again without depot‐specific differences. ADSC s from both depots differentiated poorly to chondroblasts. Gluteal ADSC s displayed significantly higher adipogenic differentiation potential than abdominal cells. Osteogenic differentiation was most pronounced in gluteal cells, whereas differentiation of abdominal ADSC s was severely impaired. Our data demonstrate a depot‐specific difference in ADSC differentiation potential with abdominal cells failing to meet the criteria of multipotent ADSC s. This finding should be taken into account in future explorations of ADSC ‐derived therapeutic strategies.