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Quantifying activity: a new assay reveals T‐cell signalling in tiny skin biopsy samples
Author(s) -
Dornmair Klaus
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12387
Subject(s) - flow cytometry , multiplex , skin biopsy , biopsy , immunology , medicine , pathology , cytometry , dermatology , biology , bioinformatics
Tissue‐invasive T cells are observed in many inflammatory dermatological diseases, but in most cases, it is not known how they were attracted, what they might recognize, and to which extent they are activated. Answering these questions is surely essential for understanding pathogeneses of the diseases. In a recent issue of Experimental Dermatology , Smith et al . showed that early signalling events in skin‐resident T cells may be investigated by multiplex immunoprecipitation flow cytometry, even if only few T cells are available from skin biopsy samples. This new technology will most likely contribute to elucidating the role of skin‐invasive T cells and to understanding the pathology of dermatological diseases.