Premium
Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders
Author(s) -
Eckl KatjaMartina,
Weindl Günther,
Ackermann Katharina,
Küchler Sarah,
Casper Ramona,
Radowski Michał R.,
Haag Rainer,
Hennies Hans Christian,
SchäferKorting Monika
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12366
Subject(s) - ichthyosis , congenital ichthyosis , barrier function , transepidermal water loss , ichthyosis vulgaris , dermatology , medicine , penetration (warfare) , filaggrin , biology , stratum corneum , pathology , microbiology and biotechnology , atopic dermatitis , operations research , engineering
The aim of this study was to assess a recently established 3D model of congenital ichthyosis, representing severe epidermal barrier function defects, for skin penetration and permeation. We have generated disease models by knock‐down of either TGM 1 or ALOXE 3 in primary human keratinocytes, and using keratinocytes and fibroblasts from patients with congenital ichthyosis. The results indicate disturbed barrier function as demonstrated by increased permeation of testosterone and caffeine particularly in TGM 1 knock‐down models compared to control models. In addition, enhanced penetration of the model dye nile red incorporated into solid lipid nanoparticles and core‐multishell nanotransporters, respectively, was evident in disease models. Thus, in vitro skin disease models reproduce differences in barrier permeability and function seen in congenital ichthyosis and pave the way to personalised disease models. Furthermore, our findings indicate that nanocarriers may be useful in new, topical therapeutic approaches for the currently very limited treatment of congenital ichthyosis.