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Autophagy induced by resveratrol suppresses α ‐ MSH ‐induced melanogenesis
Author(s) -
Kim Eun Sung,
Chang Huikyoung,
Choi Hyunjung,
Shin Ji Hyun,
Park So Jung,
Jo Yoon Kyung,
Choi Eun Sun,
Baek Seok Yun,
Kim ByungGyu,
Chang Jong Wook,
Kim Jin Cheon,
Cho DongHyung
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12337
Subject(s) - autophagy , melanosome , atg5 , microbiology and biotechnology , downregulation and upregulation , resveratrol , chemistry , organelle , melanin , tyrosinase , apoptosis , biology , biochemistry , enzyme , gene
Autophagy degrades cellular components and organelles through a cooperative process involving autophagosomes and lysosomes. Although autophagy is known to mainly regulate the turnover of cellular components, the role of autophagy in melanogenesis has not been well addressed. Here, we show that inhibition of autophagy suppresses the antimelanogenesis activity of resveratrol ( RSV ), a well‐known antimelanogenic agent. RSV strongly increased autophagy in melanocytes. However, the depletion of ATG 5 significantly suppressed RSV ‐mediated antimelanogenesis as well as RSV ‐induced autophagy in melanocytes. Moreover, suppression of ATG 5 retrieved the RSV‐mediated downregulation of tyrosinase and TRP 1 in α ‐ MSH ‐treated cells. Most importantly, electron microscopy analysis revealed that autophagosomes engulfed melanin or melanosomes after combined treatment of α ‐ MSH and RSV . Taken together, these results suggest that RSV ‐mediated autophagy regulates melanogenesis.