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Mitochondrial DNA copy number – but not a mitochondrial tandem CC to TT transition – is increased in sun‐exposed skin
Author(s) -
Gebhard Daniel,
Mahler Bettina,
Matt Katja,
Burger Katharina,
Bergemann Jörg
Publication year - 2014
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12327
Subject(s) - mitochondrial dna , transition (genetics) , pyrimidine dimer , mitochondrion , microbiology and biotechnology , oxidative stress , dna damage , mutation , biology , dna , chemistry , genetics , biochemistry , gene
Mitochondrial DNA (mt DNA ) mutations are causatively associated with photo‐ageing and are used as biomarkers of UV exposure. The most prominent mitochondrial mutation is the common deletion ( CD ), which is induced in many tissues by oxidative stress. More photo‐specific mutations might be CC to TT tandem transitions which arise from UV ‐induced cyclobutane pyrimidine dimers. As nucleotide excision repair is absent in mitochondria, this DNA damage can presumably not be repaired resulting in high mitochondrial mutation levels. Here, we analysed levels of the CD , a mitochondrial and a chromosomal tandem transition in epidermis and dermis from exposed and less UV ‐exposed skin. We also analysed mt DNA copy number, for which changes as a result of oxidative stress have been described in different experimental settings. Whereas mitochondrial tandem transition levels were surprisingly low with no discernible correlation with UV exposure, mt DNA copy number and CD were significantly increased in UV ‐exposed samples.