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Fructose 1, 6‐diphosphate regulates desmosomal proteins and collagen fibres in human skin equivalents
Author(s) -
Choi Hyun,
Yang Seung Ha,
Bae IlHong,
Park JuYearl,
Kim HyoungJune,
Noh Minsoo,
Lee Tae Ryong,
Shin Dong Wook
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12279
Subject(s) - kallikrein , human skin , chemistry , proteases , glycolysis , biochemistry , photoaging , microbiology and biotechnology , enzyme , biology , dermatology , medicine , genetics
We previously reported that fructose 1,6‐diphosphate ( FDP ), a glycolytic metabolite, alleviates ultraviolet B ‐induced oxidative skin damage. Here, we further examined the effects of FDP on skin. FDP decreased the number of desmosomes, whereas it increased collagen fibres in skin equivalents ( SE s). FDP significantly decreased the expression of corneodesmosomal components such as desmoglein 1 ( DSG 1), desmocollin 1 ( DSC 1) and corneodesmosin ( CDSN ), and desquamation‐related proteases, kallikrein 5 ( KLK 5) and kallikrein 7 ( KLK 7) in normal human epidermal keratinocytes ( NHEK s). In addition, FDP treatment increased the phosphorylation of p38 MAPK , but the decreased expression of corneodesmosomal components is not recovered by the treatment of p38 MAPK inhibitors. Interestingly, FDP diminished the amplitude of Ca 2+ fluxes through down‐regulation of SERCA 2. Taken together, these results suggested that FDP induced a decrease in desmosomes and an increase in collagen fibres similar to the process of chemical peeling, the most common treatments for ageing skin.