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Knock‐down of filaggrin does not affect lipid organization and composition in stratum corneum of reconstructed human skin equivalents
Author(s) -
Drongelen Vincent,
AlloulRamdhani Mariam,
Danso Mogbekeloluwa O.,
Mieremet Arnout,
Mulder Aat,
Smeden Jeroen,
Bouwstra Joke A.,
El Ghalbzouri Abdoelwaheb
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12271
Subject(s) - filaggrin , corneocyte , stratum corneum , loricrin , barrier function , skin equivalent , human skin , microbiology and biotechnology , keratin , chemistry , epidermis (zoology) , involucrin , biophysics , biology , atopic dermatitis , keratinocyte , biochemistry , immunology , anatomy , genetics , in vitro
Human skin mainly functions as an effective barrier against unwanted environmental influences. The barrier function strongly relies on the outermost layer of the skin, the stratum corneum ( SC ), which is composed of corneocytes embedded in an extracellular lipid matrix. The importance of a proper barrier function is shown in various skin disorders such as atopic dermatitis ( AD ), a complex human skin disorder strongly associated with filaggrin ( FLG ) null mutations, but their role in barrier function is yet unclear. To study the role of FLG in SC barrier properties in terms of SC lipid organization and lipid composition, we generated an N / TERT ‐based 3 D ‐skin equivalent ( NSE ) after knock‐down of FLG with sh RNA . In these NSE s, we examined epidermal morphogenesis by evaluating the expression of differentiation markers keratin 10, FLG , loricrin and the proliferation marker ki67. Furthermore, the SC was extensively analysed for lipid organization, lipid composition and SC permeability. Our results demonstrate that FLG knock‐down ( FLG ‐ KD ) did not affect epidermal morphogenesis, SC lipid organization, lipid composition and SC permeability for a lipophilic compound in NSE s. Therefore, our findings indicate that FLG ‐ KD alone does not necessarily affect the functionality of a proper barrier function.