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Deranged epidermal differentiation in kl/kl mouse and the effects of βKlotho si RNA on the differentiation of H aCaT cells
Author(s) -
Nakai Kozo,
Yoneda Kozo,
Haba Reiji,
Kushida Yoshio,
Katsuki Naomi,
Moriue Tetsuya,
Kosaka Hiroaki,
Kubota Yasuo,
Inoue Shigeaki
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12258
Subject(s) - loricrin , hacat , filaggrin , cellular differentiation , keratin , involucrin , keratinocyte , microbiology and biotechnology , biology , klotho , small interfering rna , gene knockdown , protein kinase a , cell culture , chemistry , kinase , transfection , endocrinology , immunology , biochemistry , gene , genetics , atopic dermatitis , kidney
Mice deficient in the klotho gene ( kl/kl mice) display the phenotypes of human ageing. We found that the expression of epidermal differentiation‐associated factors (keratin 1, keratin 10, filaggrin and loricrin) was lower in the skin of kl/kl mice than that of wild‐type mice. In vitro experiments showed that the expression of βKlotho, a family of klotho gene‐encoded protein, was induced concomitantly with the differentiation of an immortalized human epidermal keratinocyte cell line (HaCaT cells) when they were cultured in an air–liquid interface. βKlotho knockdown by small interfering ribonucleic acid suppressed the expression of the above differentiation‐associated factors in HaCaT cells. βKlotho small interfering ribonucleic acid increased the expression of keratin 14, which is expressed in mitotically active basal layer cells, and activated p44/p42 mitogen‐activated protein kinase in the HaCaT cells grown in the air–liquid interface. These findings suggest that the epidermal differentiation is deranged in kl/kl mice, and βKlotho is required for the differentiation of human epidermal keratinocytes.