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Bleomycin inhibits adipogenesis and accelerates fibrosis in the subcutaneous adipose layer through TGF ‐β1
Author(s) -
Ohgo Shiro,
Hasegawa Seiji,
Hasebe Yuichi,
Mizutani Hiroshi,
Nakata Satoru,
Akamatsu Hirohiko
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12256
Subject(s) - adipogenesis , fibrosis , adipose tissue , bleomycin , adipocyte , medicine , endocrinology , dermis , chemistry , cancer research , pathology , chemotherapy
Systemic sclerosis [scleroderma ( SS c)]‐associated skin fibrosis is characterized by increased fibrosis in the dermis and a reduction in the thickness of the subcutaneous adipose tissue layer. Although many studies have examined fibrosis in SS c, only a few studies have focused on the associated reduction in the thickness of the subcutaneous adipose tissue layer. In this study, we investigated the effects of SS c‐induced fibrosis on adipose tissue. We found that bleomycin suppresses adipogenesis in adipose‐derived stem cells ( ASC s) and stimulates ASC s to express transforming growth factor β1 ( TGF ‐β1 ), which suppresses adipogenesis and promotes fibrosis. Furthermore, we found that adipocyte‐conditioned medium suppressed collagen synthesis by fibroblasts in fibrosis‐like conditions. We concluded that in the skin affected by bleomycin‐induced fibrosis, increased TGF ‐β1 expression suppresses adipogenesis and promotes adipocyte fibrosis. It was also suggested that adipocytes have an inhibitory effect on the progression of fibrosis.