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Evidence that Notch and Delta expressions have a role in dermal condensate aggregation during wool follicle initiation
Author(s) -
Xavier Stephanie P.,
GordonThomson Clare,
Wynn Peter C.,
McCullagh Peter,
Thomson Peter C.,
Tomkins Lisa,
Mason Rebecca S.,
Moore Geoffrey P. M.
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12217
Subject(s) - mesenchymal stem cell , hair follicle , microbiology and biotechnology , follicle , primordium , notch signaling pathway , dermal papillae , chemistry , explant culture , biology , in vitro , endocrinology , signal transduction , biochemistry , gene
Notch pathway genes have been implicated in the commitment of mesenchymal cells to a wool follicle cell fate. Notch1 and D elta1 transcripts were quantified in fetal skin of fine‐woolled ( M erino) and strong‐woolled ( T ukidale) sheep at two time points: either preceding (d56) or during (d70) the first wave of follicle initiation. DIG ‐labelled probes for both transcripts were localised in the epithelium, some mesenchymal cells, and in the dermal condensates of primordia. The possibility that condensates selectively incorporated D elta1‐labelled mesenchymal cells is considered. The involvement of N otch1 in condensate formation was also explored in cultured fetal skin explants and whisker papilla cells using DAPT to block Notch signalling. In its presence, follicle initiation in skin explants was reduced, and the propensity for cultured papilla cells to aggregate was abolished. Results suggest that N otch1 activation is a prerequisite for mesenchymal aggregation. It is speculated that D elta interactions contribute to condensate formation, in vivo .