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Blue light irradiation suppresses dendritic cells activation in vitro
Author(s) -
Fischer Michael R.,
Abel Manuela,
Lopez Kostka Susanna,
Rudolph Berenice,
Becker Detlef,
Stebut Esther
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12193
Subject(s) - irradiation , stimulation , cytokine , in vitro , chemistry , flow cytometry , dendritic cell , inflammation , immunology , psoriasis , microbiology and biotechnology , medicine , cancer research , biology , immune system , endocrinology , biochemistry , physics , nuclear physics
Blue light is a UV ‐free irradiation suitable for treating chronic skin inflammation, for example, atopic dermatitis, psoriasis, and hand‐ and foot eczema. However, a better understanding of the mode of action is still missing. For this reason, we investigated whether dendritic cells ( DC ) are directly affected by blue light irradiation in vitro . Here, we report that irradiation neither induced apoptosis nor maturation of monocyte‐derived and myeloid DC . However, subsequent DC maturation upon LPS / IFN γ stimulation was impaired in a dose‐dependent manner as assessed by maturation markers and cytokine release. Moreover, the potential of this DC to induce cytokine secretion from allogeneic CD 4 T cells was reduced. In conclusion, unlike UV irradiation, blue light irradiation at high and low doses only resulted in impaired DC maturation upon activation and a reduced subsequent stimulatory capacity in allogeneic MLR s with strongest effects at higher doses.