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Resveratrol induces autophagy through death‐associated protein kinase 1 ( DAPK 1) in human dermal fibroblasts under normal culture conditions
Author(s) -
Choi Min Sik,
Kim Yoonkyung,
Jung JiYong,
Yang Seung Ha,
Lee Tae Ryong,
Shin Dong Wook
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12175
Subject(s) - resveratrol , autophagy , ampk , protein kinase a , kinase , chemistry , microbiology and biotechnology , pharmacology , biology , biochemistry , apoptosis
Abstract Autophagy is an essential process degrading damaged components. Although resveratrol has various beneficial activities for health, little is known about the effects of resveratrol on autophagy in skin. We investigated whether resveratrol affects autophagy in human dermal fibroblasts grown in complete medium. We found that after the resveratrol treatment, LC 3‐ II reached a maximum level at 8 h and then gradually decreased. By PCR array analysis, we identified death‐associated protein kinase 1 ( DAPK 1) as a new target of resveratrol, and we confirmed that the expression level of DAPK 1 was enhanced by resveratrol. We also demonstrated that DAPK 1 knock‐down by si RNA was sufficient to reduce resveratrol‐induced autophagy but did not affect the phosphorylation level of AMP ‐activated kinase ( AMPK ), a well‐known target of resveratrol. These data indicate that resveratrol‐induced autophagy can be mediated by DAPK 1, raising the possibility that some of the beneficial effects of resveratrol may be due to its regulation of DAPK 1.