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Expression analysis of multiple micro RNA s in each patient with scleroderma
Author(s) -
Koba Shigeru,
Jinnin Masatoshi,
Inoue Kuniko,
Nakayama Wakana,
Honda Noritoshi,
Makino Katsunari,
Kajihara Ikko,
Makino Takamitsu,
Fukushima Satoshi,
Ihn Hironobu
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12173
Subject(s) - scleroderma (fungus) , microrna , rna , medicine , localized scleroderma , gastroenterology , pathology , biology , gene , fibrosis , genetics , inoculation
In this study, we compared expression pattern of multiple micro RNA s in individual patient with scleroderma with that in normal subject. Serum levels of six micro RNA s (miR‐7 g, miR‐21, miR‐29b, miR‐125, miR‐145 and miR‐206) were evaluated using real‐time PCR in 15 patients with scleroderma and 15 normal subjects. While levels of the six micro RNA s were similar between the two groups, we found significant difference in the ranks between mi RNA s in patients with scleroderma. Additionally, levels of let‐7 g and miR‐125b showed strong and significant correlation in normal subjects, but not in patients with scleroderma. Thus, mi RNA expression pattern may be different in patients with scleroderma. We also found the combination of serum levels of miR‐206 and miR‐21 was more useful in distinguishing patients with scleroderma from normal subjects than either miR‐206 or miR‐21 alone. Our study is the first to demonstrate different expression profiles of multiple micro RNA s in each patient with scleroderma and examine its clinical significance.