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NADPH oxidase is a novel target of delphinidin for the inhibition of UVB ‐induced MMP ‐1 expression in human dermal fibroblasts
Author(s) -
Lim TaeGyu,
Jung Sung Keun,
Kim Jongeun,
Kim Yoona,
Lee Hyong Joo,
Jang Tae Su,
Lee Ki Won
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12157
Subject(s) - delphinidin , chemistry , apocynin , microbiology and biotechnology , nadph oxidase , western blot , phosphorylation , biochemistry , reactive oxygen species , biology , cyanidin , antioxidant , gene
We investigated the reported antiphotoaging effects of the major anthocyanidin delphidin and sought to identify its specific molecular target during UVB ‐induced MMP ‐1 expression. Delphinidin treatment significantly inhibited UVB ‐induced MMP ‐1 expression in primary cultured human dermal fibroblasts ( HDF ), an effect associated with the suppression of MKK 4‐ JNK 1/2, MKK 3/6‐p38 and MEK ‐ ERK 1/2 phosphorylation. Further investigation revealed that delphinidin significantly inhibited UVB ‐induced ROS production and NOX activity. Interestingly, the inhibitory effect of delphinidin on UVB ‐induced NOX activity was stronger than that of apocynin, a pharmaceutical NOX inhibitor. Fractioned cell analysis results using a W estern blot assay showed that this effect occurred through the inhibition of UVB ‐induced P47 phox (a NOX subunit) translocation from the cytosol to the membrane. Pull down assays demonstrated that delphinidin binds directly to P47 phox in vitro . Collectively, our results suggest that delphinidin targets NOX , resulting in the suppression of UVB ‐induced MMP ‐1 expression in human dermal fibroblasts.