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Dynamics of intracellular calcium in cultured human keratinocytes after localized cell damage
Author(s) -
Tsutsumi Moe,
Goto Makiko,
Denda Mitsuhiro
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12136
Subject(s) - scratch , epidermis (zoology) , calcium , wound healing , intracellular , calcium in biology , keratinocyte , microbiology and biotechnology , chemistry , biophysics , cell culture , anatomy , in vitro , biology , biochemistry , immunology , materials science , composite material , genetics , organic chemistry
Recovery of cultured keratinocytes after scratch damage is considered to be a wound‐healing model. In this study, we observed changes in intracellular calcium concentration ([Ca 2+ ] i ) in cultured human keratinocytes after scratch damage. Immediately after scratch damage, a wave of increased [Ca 2+ ] i radiated outward from the damaged area and then disappeared gradually. But, [Ca 2+ ] i remained elevated in a peripheral layer of cells around the damaged area for several minutes. This layer did not appear in calcium‐free medium. When the culture was switched to calcium‐free medium for 30 min immediately after scratch damage, then switched back to standard (Ca 2+ ‐containing) medium, the recovery ratio after 24 h was approximately 25% lower than that of the culture in standard medium throughout. We speculate that delineation of damage sites by a layer of cells with increased [Ca 2+ ] i might be part of a signalling pathway that appropriately directs the wound‐healing process in epidermis.