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Topical tacrolimus does not negatively impact acute skin wound healing
Author(s) -
Namkoong Sun,
Chung Jimin,
Yoo Jiyeon,
Jung Minyoung,
Gye Jiwon,
Kim Ji Seok,
Kim Jee Young,
Ahn Sung Ku,
Park Byung Cheol,
Kim Myung Hwa,
Hong Seung Phil
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12131
Subject(s) - tacrolimus , wound healing , medicine , hairless , clobetasol propionate , pharmacology , dermatology , corticosteroid , surgery , transplantation , chemistry , biochemistry
Despite the increasing use of topical tacrolimus, there is little information about its effect on skin wound healing. To determine effects on acute cutaneous wound healing, two full‐thickness skin wounds were imparted on the backs of 45 hairless mice, which were then divided into vehicle‐, topical tacrolimus‐ and topical steroid‐treated group. Each drug was topically applied once daily. The wound area was assessed by using dermoscopic images every two days after wounding. At 3, 7 and 11 days after wounding, 10 wounds in each group were collected for semi‐quantitative analysis of histological features including re‐epithelialization, polymorphonuclear leucocytes, fibroblasts and collagen. We also checked the mRNA expression levels of EGF , TGF ‐β, TNF ‐α and IL ‐1α. While topical application of clobetasol propionate was found to delay re‐epithelialization and infiltration of polymorphonuclear leucocyte, topical treatment with tacrolimus showed patterns similar to that of the vehicle. In the tacrolimus‐treated group, mRNA expression levels of IL ‐1α and TGF ‐β were slightly decreased, while the others were similar with the vehicle‐treated group. Unlike steroid, topical tacrolimus, therefore, did not disturb the wound healing process in a murine skin wound model.