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The sound of silence: autosomal recessive congenital ichthyosis caused by a synonymous mutation in ABCA 12
Author(s) -
Goldsmith Tomer,
FuchsTelem Dana,
Israeli Shirli,
Sarig Ofer,
PadalonBrauch Gilly,
Bergman Reuven,
Indelman Margarita,
Sprecher Eli,
Nousbeck Janna
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12110
Subject(s) - genetics , ichthyosis , exon , biology , congenital ichthyosis , gene , mutation
Autosomal recessive congenital ichthyosis refers to a heterogeneous group of cornification disorders of major impact on patients’ life. The disease has been linked so far to mutations in 8 distinct genes. We report a consanguineous family of A rab M uslim origin with several members displaying a severe form of congenital ichthyosiform erythroderma. Using a panel of polymorphic microsatellite markers, we identified a region of homozygosity shared by all patients on 2q34, in a region harbouring the ABCA 12 gene. Direct sequencing of genomic DNA derived from a patient failed to reveal any obviously pathogenic change in the coding sequence of this gene. In contrast, cDNA sequence analysis revealed the existence of a 163‐bp‐long deletion in exon 24, thus pointing to a splicing defect. Careful reanalysis of the genomic DNA sequence revealed apart from several known single‐nucleotide polymorphisms, a hitherto unreported homozygous synonymous mutation in exon 24 (c.3456 G > A ; p. S 1152S), which was found to lead to the formation of a novel splicing acceptor site. Synonymous mutations have been shown to uncommonly cause inherited disorders in humans. Here, we present the first example of a congenital form of ichthyosis resulting from such a genetic defect.