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Two‐photon microscopy of dermal innervation in a human re‐innervated model of skin
Author(s) -
Sevrain David,
Grand Yann,
Buhé Virginie,
Jeanmaire Christine,
Pauly Gilles,
Carré JeanLuc,
Misery Laurent,
Lebonvallet Nicolas
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12108
Subject(s) - dermis , two photon excitation microscopy , anatomy , microscopy , sensory system , fluorescence microscope , pathology , human skin , chemistry , biology , neuroscience , medicine , fluorescence , optics , physics , genetics
When skin is injured, innervation can be severely disrupted. The subsequent re‐innervation processes are poorly understood notably because of the inability to image the full meandering course of nerves with their ramifications and endings from histological slices. In this letter, we report on two‐photon excitation fluorescence ( TPEF ) microscopy of entire human skin explants re‐innervated by rodent sensory neurons labelled with the styryl dye FM 1‐43. TPEF imaging of nerve fibres to a depth up to roughly 300 μm within the dermis was demonstrated, allowing three‐dimensional reconstruction of the neural tree structure. Endogenous second‐harmonic imaging of type I fibrillar collagen was performed in parallel to TPEF imaging using the same nonlinear microscope, revealing the path of the nerves through the dermis.