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Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture
Author(s) -
Hill Rebecca P.,
Gardner Aaron,
Crawford Heather C.,
Richer Rachel,
Dodds Anna,
Owens William A.,
Lawrence Clifford,
Rao Sam,
Kara Bo,
James S. Elizabeth,
Jahoda Colin A.
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12107
Subject(s) - dermal papillae , keratinocyte , hair follicle , microbiology and biotechnology , dermal fibroblast , keratinocyte growth factor , fibroblast , human skin , transplantation , regeneration (biology) , chemistry , cell culture , biology , fibroblast growth factor , in vitro , receptor , medicine , biochemistry , genetics
Traditional skin grafting techniques are effective but limited methods of skin replacement. Autologous transplantation of rapidly cultured keratinocytes is successful for epidermal regeneration, but the current gold‐standard technique requires mouse fibroblast feeders and serum‐rich media, with serum‐free systems and dermal fibroblast ( DF ) feeders performing relatively poorly. Here, we investigated the capacity of human hair follicle dermal cells to act as alternative supports for keratinocyte growth. Dermal papilla ( DP ) dermal sheath ( DS ), DF and 3 T 3 cells were used as inactivated feeder cells for human keratinocyte coculture. Under conditions favouring dermal cells, proliferation of keratinocytes in the presence of either DS or DP cells was significantly enhanced compared with DF cells, at levels comparable to keratinocytes cultured under gold‐standard conditions. Secreted protein acidic and rich in cysteine ( SPARC ) expression increased DS and DP cells relative to DF s; however, further experiments did not demonstrate a role in keratinocyte support.