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Wound‐associated macrophages control collagen 1α2 transcription during the early stages of skin wound healing
Author(s) -
Rodero Mathieu P.,
Legrand Julien M. D.,
BouGharios George,
Khosrotehrani Kiarash
Publication year - 2013
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12068
Subject(s) - wound healing , fibrosis , in vivo , regeneration (biology) , macrophage , transcription (linguistics) , microbiology and biotechnology , chemistry , medicine , pathology , immunology , biology , in vitro , biochemistry , linguistics , philosophy
Wound‐associated fibrosis is important to provide tensile strength upon wound healing but at the same time is detrimental to proper tissue regeneration. To date, there is no clear evidence of the role of macrophages and their subpopulations in the control of the kinetics of collagen production during wound healing. To evaluate in vivo the contribution of macrophages in collagen transcription, we depleted macrophages after wounding luciferase reporter mice of the collagen 1 alpha 2 ( C ol 1α2) promoter activity. Our data reveal that C ol 1α2 starts to be transcribed at D 2 after wounding, reaching a plateau after 7 days. Sustained macrophage depletion significantly reduced collagen 1α2 transcription from D 4, indicating that the control of fibrosis by macrophages occurs during the early stages of the wound healing process. In conclusion, our results demonstrate an important role of wound macrophages in the control of collagen production during wound healing.