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Epidermal morphogenesis during progressive in vitro 3 D reconstruction at the air–liquid interface
Author(s) -
Frankart Aurélie,
Malaisse Jérémy,
Vuyst Evelyne,
Minner Frédéric,
Rouvroit Catherine Lambert,
Poumay Yves
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12020
Subject(s) - epidermis (zoology) , keratinocyte , microbiology and biotechnology , morphogenesis , in vitro , barrier function , human skin , biology , cellular differentiation , in vivo , regeneration (biology) , biochemistry , anatomy , genetics , gene
Keratinocyte monolayers, cultured in immersed conditions, constitute a frequently used in vitro model system to study keratinocytes behaviour in response to environmental assaults. However, monolayers lack the keratinocyte terminal differentiation and the organization of the epidermal tissue, which are observed in vivo . Advancements of in vitro techniques were used to reconstruct three‐dimensional equivalents that mimic human epidermis in terms of layering, differentiation and barrier function. Here, we update a published method and illustrate the progressive morphogenesis responsible for in vitro reconstruction. The analysis of cell proliferation, expression of differentiation markers and barrier efficacy demonstrate the excellent similarity of the reconstructed tissue with normal human epidermis. Availability of epidermal tissue during its reconstruction phase in culture appears crucial for studies intending to challenge the barrier function.