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Expression and localization of A rtemis serine 516 phosphorylation in human scalp skin
Author(s) -
Wu XianJie,
Jing Jing,
Zhu JianWei,
Xue Dan,
Liu Hai,
Böhm Markus,
Lu ZhongFa,
Zheng Min
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12009
Subject(s) - inner root sheath , stratum granulosum , phosphorylation , outer root sheath , human skin , epidermis (zoology) , biology , hair follicle , scalp , microbiology and biotechnology , serine , cancer research , stratum corneum , pathology , anatomy , medicine , genetics
Abstract Artemis phosphorylation at serine 516 ( S er516) has important regulatory functions in the repair of radiation‐induced DNA damage, V ( D ) J recombination, p53‐dependent apoptosis and cell cycle control. Accordingly, A rtemis mutations can lead to O menn syndrome, which is associated with human radiosensitive severe combined immunodeficiency syndrome and alopecia. In this study, we investigated the expression of S er516 phosphorylation of Artemis in the epidermis and epidermal appendages in normal human scalp skin. Immunofluorescence analysis revealed S er516 phosphorylation of A rtemis in the upper and middle portion of anagen hair follicle [including outer root sheath ( ORS ), inner root sheath but not stratum basale], hair matrix, sebaceous glands (secretory and ductal portions), eccrine sweat glands (secretory and ductal portions) and epidermis (stratum basale and stratum granulosum), respectively. Artemis phosphorylation at S er516 was most prominent in ORS keratinocytes. Therefore, we suggest that phosphorylation of A rtemis at S er516 could be involved in regulation of human epidermal appendages.