Premium
Lack of micro RNA miR‐150 reduces the capacity of epidermal L angerhans cell cross‐presentation
Author(s) -
Mi QingSheng,
Xu YingPing,
Qi RuiQun,
Shi YuLing,
Zhou Li
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12008
Subject(s) - microrna , microbiology and biotechnology , biology , function (biology) , langerhans cell , cd8 , cross presentation , antigen presentation , phenotype , t cell , gene , antigen , immunology , genetics , immune system
Micro RNA s (mi RNA s) are evolutionarily conserved small non‐coding RNA s that repress target genes at post‐transcriptional level. Langerhans cells ( LC s) are skin‐residential dendritic cells ( DC s) with a life cycle distinct from other types of DC s. mi RNA deficiency interrupts the homoeostasis and function of epidermal LC s, suggesting the critical roles of mi RNA s in LC development and function. However, the roles of individual mi RNA s in regulating LC development and function remain completely unknown. MiRNA miR‐150 is highly expressed in mature lymphocytes and regulates T ‐ and B ‐cell development and function. Here, we reported that miR‐150 is also expressed in epidermal LC s, and its expression is significantly down‐regulated during in vitro LC maturation. Using a mi R ‐150 knockout mouse model, we found that lack of mi R ‐150 reduces the capacity of LC s to cross‐present a soluble antigen to antigen‐specific CD 8 + T cells, but does not disturb the development, maturation, migration and phagocytic capacity of LC s. Thus, our data indicate that miR‐150 is required for LC cross‐presentation.