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Morphogenesis of chimeric hair follicles in engineered skin substitutes with human keratinocytes and murine dermal papilla cells
Author(s) -
Sriwiriyat Penkanok,
Lynch Kaari A.,
Maier Elizabeth A.,
Hahn Jennifer M.,
Supp Dorothy M.,
Boyce Steven T.
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12003
Subject(s) - dermal papillae , hair follicle , green fluorescent protein , human skin , microbiology and biotechnology , keratinocyte , chemistry , epidermis (zoology) , dermis , morphogenesis , biology , in vitro , anatomy , biochemistry , gene , genetics
Engineered skin substitutes ( ESS ) have been used successfully to treat life‐threatening burns, but lack cutaneous appendages. To address this deficiency, dermal constructs were prepared using collagen‐glycosaminoglycan scaffolds populated with murine dermal papilla cells expressing green fluorescent protein ( mDPC ‐ GFP ), human dermal papilla cells ( hDPC ) and/or human fibroblasts ( hF ). Subsequently, human epidermal keratinocytes ( hK ) or hK genetically modified to overexpress stabilized β‐catenin ( hK ') were used to prepare ESS epithelium. After 10 days incubation at air–liquid interface, ESS were grafted to athymic mice and were evaluated for 6 weeks. Neofollicles were observed in ESS containing mDPC ‐ GFP , but not hDPC or hF , independent of whether or not the hK were genetically modified. Based on detection of GFP fluorescence, mDPC were localized to the dermal papillae of the well‐defined follicular structures of grafted ESS. In addition, statistically significant increases in LEF1 , WNT10A and WNT10B were found in ESS with neofollicles. These results demonstrate a model for generation of chimeric hair in ESS .