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The molecular origin and evolution of dim‐light vision in mammals
Author(s) -
Bickelmann Constanze,
Morrow James M.,
Du Jing,
Schott Ryan K.,
Hazel Ilke,
Lim Steve,
Müller Johannes,
Chang Belinda S. W.
Publication year - 2015
Publication title -
evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.84
H-Index - 199
eISSN - 1558-5646
pISSN - 0014-3820
DOI - 10.1111/evo.12794
Subject(s) - biology , rhodopsin , evolution of mammals , amniote , evolutionary biology , visual phototransduction , myr , clade , molecular evolution , vertebrate , phylogenetics , most recent common ancestor , convergent evolution , zoology , retinal , genetics , gene , genome , botany
The nocturnal origin of mammals is a longstanding hypothesis that is considered instrumental for the evolution of endothermy, a potential key innovation in this successful clade. This hypothesis is primarily based on indirect anatomical inference from fossils. Here, we reconstruct the evolutionary history of rhodopsin—the vertebrate visual pigment mediating the first step in phototransduction at low‐light levels—via codon‐based model tests for selection, combined with gene resurrection methods that allow for the study of ancient proteins. Rhodopsin coding sequences were reconstructed for three key nodes: Amniota, Mammalia, and Theria. When expressed in vitro, all sequences generated stable visual pigments with λ MAX values similar to the well‐studied bovine rhodopsin. Retinal release rates of mammalian and therian ancestral rhodopsins, measured via fluorescence spectroscopy, were significantly slower than those of the amniote ancestor, indicating altered molecular function possibly related to nocturnality. Positive selection along the therian branch suggests adaptive evolution in rhodopsin concurrent with therian ecological diversification events during the Mesozoic that allowed for an exploration of the environment at varying light levels.