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FUNCTIONAL GENOMICS OF ADAPTATION TO HYPOXIC COLD‐STRESS IN HIGH‐ALTITUDE DEER MICE: TRANSCRIPTOMIC PLASTICITY AND THERMOGENIC PERFORMANCE
Author(s) -
Cheviron Zachary A.,
Connaty Alex D.,
McClelland Grant B.,
Storz Jay F.
Publication year - 2014
Publication title -
evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.84
H-Index - 199
eISSN - 1558-5646
pISSN - 0014-3820
DOI - 10.1111/evo.12257
Subject(s) - biology , transcriptome , peromyscus , adaptation (eye) , hypoxia (environmental) , phenotypic plasticity , plasticity , deer mouse , ecology , evolutionary biology , gene , genetics , gene expression , oxygen , neuroscience , chemistry , physics , organic chemistry , thermodynamics
In species that are distributed across steep environmental gradients, adaptive variation in physiological performance may be attributable to transcriptional plasticity in underlying regulatory networks. Here we report the results of common‐garden experiments that were designed to elucidate the role of regulatory plasticity in evolutionary adaptation to hypoxic cold‐stress in deer mice ( Peromyscus maniculatus ). We integrated genomic transcriptional profiles with measures of metabolic enzyme activities and whole‐animal thermogenic performance under hypoxia in highland (4350 m) and lowland (430 m) mice from three experimental groups: (1) wild‐caught mice that were sampled at their native elevations; (2) wild‐caught/lab‐reared mice that were deacclimated to low‐elevation conditions in a common‐garden lab environment; and (3) the F 1 progeny of deacclimated mice that were maintained under the same low‐elevation common‐garden conditions. In each experimental group, highland mice exhibited greater thermogenic capacities than lowland mice, and this enhanced performance was associated with upregulation of transcriptional modules that influence several hierarchical steps in the O 2 cascade, including tissue O 2 diffusion (angiogenesis) and tissue O 2 utilization (metabolic fuel use and cellular oxidative capacity). Most of these performance‐related transcriptomic changes occurred over physiological and developmental timescales, suggesting that regulatory plasticity makes important contributions to fitness‐related physiological performance in highland deer mice.