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Differential Expression of micro RNA s in Equine Sarcoids: Preliminary Results from an In Vitro Model
Author(s) -
TerronCanedo N.,
Britton C.,
Nicolson L.,
Nasir L.
Publication year - 2014
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.12323_32
Subject(s) - rna , cell culture , in vitro , non coding rna , cell , biology , microbiology and biotechnology , cancer research , gene , genetics
Reasons for performing the study Equine sarcoids are locally aggressive fibroblastic cutaneous tumours, representing the third most common health problem in working horses. In developed countries they also have welfare and economic impact (e.g. prepurchase examination). No current treatment offers 100% success rate. It is now well established that Bovine Papillomavirus ( BPV ) types 1 and 2 play an important role in the pathogenesis of sarcoid tumours. In a number of human cancers, aberrant expression of micro RNA s has been linked to the cancerous phenotype. micro RNA s are small noncoding RNAs that regulate essential biological and cellular processes. Currently there is much interest in their role as biomarkers and potential therapeutic agents for many diseases. Objectives In an attempt to improve our understanding of the pathobiology of sarcoids, we aim to identify specific micro RNAs associated with equine sarcoids. Study design Differential expression of micro RNA s was assessed in control equine fibroblasts ( E q P alfs) and E q P alfs transformed with the BPV ‐1 genome ( S 6‐2s). Methods Using a commercially available human microarray, 453 micro RNA s were interrogated in RNA samples from both cell lines. Each cell line was analysed in triplicate. Results 219 individual micro RNA s were differentially expressed in control ( E q P alFs) versus BPV ‐transformed cells ( S 6‐2s) (P<0.05). of these, 148 micro RNA s (68%) were underexpressed in S 6‐2 cells relative to E q P alfs. Conclusions We have shown that BPV ‐1 transformed equine cell lines exhibit a different micro RNA profile to control equine fibroblasts and the aberrant expression of micro RNA s in S 6‐2 cells can be attributed to the presence of BPV ‐1 genomes. Our future work will focus on studying the expression profiles of a subset of micro RNA s in a larger panel of cell lines and sarcoid tumours to help elucidate the significance of the deregulated expression. Ethical animal research: Local ethical approval was granted to conduct this study. Sources of funding: This work is funded by the Horserace Betting Levy Board with contribution from the Vet Trust Fund ( J ames H erriot Scholarship). Competing interests: None.