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Characterisation of nasal S taphylococcus delphini and S taphylococcus pseudintermedius isolates from healthy donkeys in T unisia
Author(s) -
Gharsa H.,
Slama K. Ben,
GómezSanz E.,
Gómez P.,
Klibi N.,
Zarazaga M.,
Boudabous A.,
Torres C.
Publication year - 2015
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.12305
Subject(s) - staphylococcus pseudintermedius , multilocus sequence typing , biology , microbiology and biotechnology , fusidic acid , pulsed field gel electrophoresis , tetracycline , staphylococcus , staphylococcus intermedius , staphylococcus aureus , genotype , bacteria , antibiotics , gene , genetics
Summary Reasons for performing study S taphylococcus intermedius group ( SIG ) bacteria can colonise the nares of some animals but are also emerging pathogens in humans and animals. Objectives To analyse SIG nasal carriage in healthy donkeys destined for food consumption in T unisia and to characterise recovered isolates. Methods Nasal swabs from 100 healthy donkeys were tested for SIG recovery, and isolates were identified by biochemical and molecular methods. Antimicrobial susceptibility of isolates was tested and detection of antimicrobial resistance and virulence genes was performed. Isolates were typed at the clonal level by multilocus sequence typing and Sma I pulsed‐field gel electrophoresis. Results S taphylococcus delphini and S taphylococcus pseudintermedius (included in SIG ) were obtained in 19% and 2% of the tested samples, respectively, and one isolate per sample was characterised. All isolates were meticillin susceptible and mecA negative. Most S . delphini and S . pseudintermedius isolates showed susceptibility to all antimicrobials tested, with the exception of 2 isolates resistant to tetracycline ( tet ( M ) gene) or fusidic acid. The following toxin genes were identified (percentage of isolates): lukS‐ I (100%), lukF‐ I (9.5%), siet (100%), se‐int (90%), sec canine (19%) and expA (9.5%). Thirteen different pulsed‐field gel electrophoresis profiles were identified among the 21 SIG isolates. Additionally, the following 9 different sequence types ( STs ) were detected by multilocus sequence typing, 6 of them new: ST219 (6 isolates), ST12 (5 isolates), ST220 (3 isolates), ST13 , ST50 , ST193 , ST196 , ST218 and ST221 (one isolate each). Conclusions S taphylococcus delphini and S . pseudintermedius are common nasal colonisers of donkeys, generally susceptible to the antimicrobials tested; nevertheless, these SIG isolates contain virulence genes, including the recently described exfoliative gene ( expA ) and several enterotoxin genes, with potential implications for public health. This is the first description of S . delphini in T unisia. The Summary is available in Chinese – see Supporting information .