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Oil Supplementation Produces an Increase in Antioxidant Biomarkers in Four‐Beat Gaited Horses
Author(s) -
Melo S,
Wanderley E,
Diniz I,
Manso H,
Manso Filho H
Publication year - 2014
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.12267_98
Subject(s) - hematocrit , uric acid , creatinine , oxidative stress , vitamin e , antioxidant , vitamin c , hemoglobin , medicine , ascorbic acid , zoology , blood urea nitrogen , lipid peroxidation , urea , glutathione peroxidase , endocrinology , chemistry , biochemistry , superoxide dismutase , food science , biology
Marcha is typically a low intensity/medium duration exercise that has the potential to induce oxidative stress. The objective of the present study was to test the hypothesis that supplementation with oil would alter biomarkers of oxidative stress in four‐beat gaited horses during marcha training. Methods Eleven gaited horses underwent training for 60 min (∼60% consisting of marcha at ∼3.5 m/s) 3 d/wk for eight weeks. Subjects were fed elephant grass (∼15 kg/day) and concentrate (∼5 kg/day). They were also supplemented with 300 mL of oil per os (vitamin E : 300 IU /kg, omega‐3: 300 g/kg; omega‐6: 80 g/kg) and had free access to water and salt. Blood samples were collected before and after four and eight weeks of supplementation for the measurement of GPx , SOD , RBC , hemoglobin, hematocrit, WBC , TPP , urea, creatinine and uric acid. ANOVA and Tukey tests were used for statistical analysis (P<0.05). Results Increases in GPx (∼12%), SOD (∼51%) and uric acid (∼19%) were observed after 8 week of supplementation (P<0.05). There were changes (P<0.05) in [urea] and [creatinine]; however, there were no changes (P>0.05) in RBC , hemoglobin, hematocrit or WBC . Published research has demonstrated that administration of supplements with antioxidants enhances the antioxidant system and protects against oxidative stress during exercise. Supplementation with oil, rich in vitamin E , omega‐3 and ‐6, produced a beneficial increase in antioxidant capacity in gaited horses during training. Conclusions The elevation in [ GPx ], [ SOD ] and [uric acid] in the present study offers a strategy to counterbalance the physiologic processes that overwhelm endogenous antioxidants during training. Ethical Animal Research Methods were approved by UFRPE Animal Care U nit (#026/2013). Sources of funding: INTEGRALMIX Animal Nutrition ( F ortaleza‐ CE ), Haras Cascatinha ( C amaragibe‐ PE ) and CAPES . Competing interests: none.