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Accuracy of a Point‐of‐Care Lactate Analyzer for Measuring Blood and Plasma L ‐Lactate Concentrations in Exercising Standardbreds
Author(s) -
Constable P,
Sulimai N,
Tinkler S,
Ko J,
Couetil L
Publication year - 2014
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.12267_61
Subject(s) - lactic acid , lactate dehydrogenase , blood lactate , anaerobic exercise , chromatography , chemistry , blood plasma , medicine , biochemistry , biology , blood pressure , enzyme , heart rate , physiology , genetics , bacteria
The Accutrend® Lactate (formerly Accusport®) is a low cost point‐of‐care L ‐lactate analyser. The analyser is widely used in the training of horses and has been used for research studies and to assist the clinical management of critically ill horses. The analyser is reported to be linear from 0.8–22.0 mmol/ L (blood) and 0.7–26.0 mmol/ L (plasma); however, test performance at high L ‐lactate concentrations in equine blood and plasma has not been well documented. The purpose of this study was to determine the accuracy of the Accutrend® Lactate analyzer over a wide range of L ‐lactate concentrations in paired blood and plasma samples. Methods Duplicate jugular blood samples were collected from 8 trained Standardbreds before, during, and after a simulated race on a treadmill into 4 mL partially evacuated tubes containing lithium heparin. Blood and plasma L ‐lactate concentrations were measured using a colorimetric lactate‐oxidase mediated reaction (BM‐Lactate® test strips and Accutrend® Lactate) and the reference enzymatic method ( L ‐lactic dehydrogenase spectrophotometry at 340 nm using samples deproteinized with 8% perchloric acid). Data was analysed using Deming regression and Bland‐Altman plots adjusted for repeated measures (P<0.05). Results The reference method measured a wide range of L ‐lactate concentrations in 128 blood (0.2–23.8 mmol/ L ) and 127 plasma (0.3–41.1 mmol/ L ) samples. The Accutrend® Lactate analyzer provided an accurate method of L ‐lactate measurement, but only when blood and plasma L ‐lactate concentrations were <12.5 mmol/ L (79% of samples) and <15.0 mmol/ L (64% of samples), respectively. Conclusions Blood L ‐lactate concentrations >12.5 mmol/ L and plasma L ‐lactate concentrations >15.0 mmol/ L measured by the Accutrend® Lactate analyzer underestimate the true value by a variable amount when compared with the enzymatic reference method. Ethical Animal Research The study was approved by the Institutional Animal Care and Use Committee at Purdue University. Sources of funding: Supported, in part, by a grant from the Indiana Horse Racing Commission. Competing interests: none.