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Preliminary Results on CD 147 Expression in Red Blood Cells of Arabian Horses
Author(s) -
Regatieri IC,
Almeida M LM,
Teixeira Neto AR,
Ferraz GC,
QueirozNeto A
Publication year - 2014
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.12267_55
Subject(s) - horse , band 3 , solute carrier family , anaerobic exercise , red blood cell , acidosis , homeostasis , transporter , anion gap , blot , metabolic acidosis , blood lactate , anaerobic glycolysis , chemistry , zoology , andrology , microbiology and biotechnology , medicine , biochemistry , biology , metabolism , glycolysis , membrane protein , membrane , gene , physiology , paleontology , heart rate , blood pressure
During anaerobic glycolysis for energy production, lactate and H + ions accumulate in muscle fibers and pass into the bloodstream. Monocarboxylate transporter type 1 and its ancillary protein CD 147 transport H + and lactate ions from the plasma into red blood cells, thereby maintaining acid/base homeostasis and retarding systemic acidosis and fatigue. The aim of this study was to compare the levels of CD 147 protein in the red blood cells of Arabian horses with different levels of athletic ability. Methods Blood samples were taken from 12 Arabian horses before exercise. The horses were divided into two groups: untrained horses ( UH , n = 6) and 160‐km endurance horses ( FEI , n = 6). The amount of CD 147 in the plasma membranes of their red blood cells was determined by Western blotting analysis using a human‐specific anti‐ CD 147 antibody previously validated for horses. The medians were compared by Mann‐Whitney Rank Sum test at 5%. Results CD 147 protein with a molecular mass of approximately 45 kDa was found in the red blood cell membranes of the studied horses. The CD 147 expression levels of the two groups were not different (P = 0.065). The observed median values (min–max) were 0.50 (0.37–1.26) and 0.27 (0.09–0.69) arbitrary optical density units for the FEI and UH group respectively. Conclusions Studies must be performed with more horses to determine if a different level of CD 147 expression exists in FEI horses related to their intensive training programs or to the genetic traits of each horse. Ethical Animal Research All procedures were approved by the institutional animal care and use committee of the university ( CEUA , Process: 019314/13). E xplicit owner informed consent for participation in this study is not stated. Sources of funding: FAPESP ‐ F undação de A mparo à P esquisa do E stado de São Paulo. Process: 2012/24193‐0. Competing interests: none.