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A Pilot Study on Genetic Speed Markers in T urkish T horoughbreds
Author(s) -
Gür E,
Onmaz AC,
Steinborn R,
Hoven R
Publication year - 2014
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.12267_102
Subject(s) - single nucleotide polymorphism , genotype , genetics , snp , biology , agarose gel electrophoresis , microbiology and biotechnology , gene
The 227‐bp SINE insertion in the promoter region of the MSTN gene, the intronic single nucleotide polymorphism ( SNP ) of MSTN , g.66493737C>T, and the SNPs BIEC 2‐417274 and BIEC 2‐417495 located upstream and downstream of MSTN seem appropriate QTL markers for racing performance of elite T horoughbreds. This pilot study aimed to find if these markers of racing ability can be used to optimize the management of the racing careers of Turkish T horoughbreds. Methods Hair roots were used for DNA extraction. The promoter insertion was analysed by agarose gel electrophoresis of the PCR product. The SNPs were typed by the amplification refractory mutation system‐real‐time PCR . Best racing distance was defined as the distance of the races in which the horse obtained the best results. Results were semi quantified by a point score based on wining and places. Results BIEC 2‐417274, BIEC 2‐417495 SNP variants and the 227‐bp SINE were strongly associated with g.66493737C>T. For the latter, the genotype frequencies were: C / C 0.23, C / T 0.52 and T / T 0.25. In total 39% of the Turkish races are distances >1600 m. Analysis of the racing career showed that both the sprinters ( C / C ) and the medium distance horse ( C / T ) were not entered optimally in races that fit with their genetic ability. Conclusions Turkish race programing should create more opportunities for the g.66493737 C /T genotypes to show their ability. This implies that more 1700 to 2800 m races should be scheduled in the racing calendar. Ethical Animal Research Owner informed consent was obtained. Sources of funding: Jockey Club of T urkey. Competing interests: none.