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Evaluation of mesenchymal stem cell migration after equine tendonitis therapy
Author(s) -
Carvalho A. M.,
Yamada A. L. M.,
Golim M. A.,
Álvarez L. E. C.,
Hussni C. A.,
Alves A. L. G.
Publication year - 2014
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.12173
Subject(s) - mesenchymal stem cell , peripheral blood mononuclear cell , medicine , in vivo , flow cytometry , pathology , adipose tissue , tendonitis , cell therapy , stem cell , tendon , in vitro , immunology , biology , microbiology and biotechnology , biochemistry
Summary Reasons for performing study Mesenchymal stem cells ( MSC s) have been used to treat equine tendonitis with promising results; however, little is known about the potential migration of these cells. Objectives To assess the possible migration of MSC s from an implantation site in the superficial digital flexor tendon ( SDFT ) to a lesion in the SDFT of the contralateral limb. Study design In vivo experimental study. Methods Adipose‐derived MSCs were isolated from 4 healthy horses. Lesions were induced in the SDFT s of both forelimbs, followed by intralesional implantation of autologous adipose‐derived MSC s labelled with nanocrystals into one of the limbs. Flow cytometry of the peripheral blood mononuclear cells and fluorescence microscopy of biopsies of the SDFT lesions were used to search for the labelled cells. Results Labelled cells were detected among the peripheral blood mononuclear cells in all animals, but labelled cells were present only in the SDFT s that were treated with the intralesional implants. Conclusion Nanocrystals were a valuable in vivo marker of MSC s to be used for tendonitis treatment. Although migration of MSC s to the bloodstream was observed, it was not possible to identify the labelled cells in the untreated tendons.