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Systemic and respiratory oxidative stress in the pathogenesis and diagnosis of R hodococcus equi pneumonia
Author(s) -
Crowley J.,
Po E.,
Celi P.,
Muscatello G.
Publication year - 2013
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.12166
Subject(s) - exhaled breath condensate , oxidative stress , pneumonia , medicine , pathogenesis , respiratory system , gastroenterology , hematology , inflammation , immunology , pathology , asthma
Summary Reasons for performing study Oxidative stress ( OS ) is most simply defined as an imbalance between oxidants and antioxidants. Oxidative stress has been suggested to play roles in various equine respiratory diseases and the significance of OS in the pathogenesis of R hodococcus equi pneumonia is unknown. Objectives To measure and relate biomarkers of OS to lesions consistent with R. equi pneumonia. Study design Case–control study. Methods Various OS biomarkers were measured from blood and exhaled breath condensate ( EBC ) samples collected from 26 foals between 1 and 2 months of age (n = 12 cases and n = 14 controls) on 2 Thoroughbred farms endemically affected by R. equi pneumonia. Foals were defined as cases (positive) or controls (negative) based on ultrasonographic evidence of pulmonary abscessation (>15 mm in diameter). Haematology and biochemistry testing was also performed on blood samples collected from the foals. Comparison of biomarkers and key haematological and biochemical markers of inflammation between the groups was performed using 2 sample t tests. Results Derivatives of reactive oxygen metabolites (d‐ ROMs ) were significantly greater in case foals than in control foals (P = 0.027) and the oxidative stress index ( OSI ) was higher in case foals (P = 0.014). Hydrogen peroxide (H 2 O 2 ) concentrations in EBC were significantly greater in case foals than in control foals (P = 0.002). Meanwhile, there were no significant differences in traditional measures of inflammation between the 2 groups. Conclusions Measuring OS in both blood and EBC provided useful information in the early diagnosis of R. equi pneumonia.