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Practical guide to single layer centrifugation of stallion semen
Author(s) -
Morrell J. M.,
Nunes M. M.
Publication year - 2018
Publication title -
equine veterinary education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.304
H-Index - 31
eISSN - 2042-3292
pISSN - 0957-7734
DOI - 10.1111/eve.12658
Subject(s) - artificial insemination , semen , insemination , sperm , sperm washing , centrifugation , medicine , andrology , intrauterine insemination , gynecology , obstetrics , pregnancy , biology , pregnancy rate , genetics , biochemistry
Summary The technique of single layer centrifugation through species‐specific colloids has been gaining acceptance over the last few years as a practical method of improving the sperm quality of stallion semen for artificial insemination. This article explains the background to the technique and the different colloid formulations used, suggests some applications of the technique with particular reference to the equine breeding industry, describes the method in detail, and finally provides some guidance for problem solving. It is intended as a practical guide for those considering using the technique to improve sperm quality and pregnancy rates in equine artificial insemination and embryo transfer.