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Identification of Corynebacterium pseudotuberculosis isolated from muscular abscesses in two horses: First report in Mexico
Author(s) -
MuñozBucio A. V.,
CortésPérez Y. A.,
ArellanoReynoso B.,
HernándezGil M.,
HernándezCastro R.,
DíazAparicio E.
Publication year - 2017
Publication title -
equine veterinary education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.304
H-Index - 31
eISSN - 2042-3292
pISSN - 0957-7734
DOI - 10.1111/eve.12585
Subject(s) - biovar , corynebacterium pseudotuberculosis , rpob , genbank , horse , microbiology and biotechnology , equidae , phylogenetic tree , rhodococcus equi , isolation (microbiology) , biology , corynebacterium , polymerase chain reaction , veterinary medicine , medicine , gene , 16s ribosomal rna , genetics , bacteria , virulence , paleontology
Summary Two Quarter Horse stallions from the same region in Mexico presented with pectoral abscesses and lameness. Exudate samples were taken from drained muscular abscesses and Corynebacterium pseudotuberculosis , identified by bacteriological techniques and multiplex polymerase chain reaction, was obtained in a pure isolation culture from both samples. The sequences of a hypervariable region of the rpoB gene showed 100% and 99% identity to C. pseudotuberculosis 316 biovar equi ( CP 003077.1) isolated from a horse. Both strains' sequences were located in the same phylogenetic cluster as other biovar equi strain sequences obtained from GenBank. Both horses responded satisfactorily to treatment, which included surgical procedures and wound management. These study cases proved the infection of Mexican horses with C. pseudotuberculosis ; however, the prevalence of this pathogen remains unknown.