Open AccessContrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: implications for vector control
Author(s) -
Rašić Gordana,
Schama Renata,
Powell Rosanna,
Macielde Freitas Rafael,
EndersbyHarshman Nancy M.,
Filipović Igor,
Sylvestre Gabriel,
Máspero Renato C.,
Hoffmann Ary A.
Publication year - 2015
Publication title -
evolutionary applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.776
H-Index - 68
ISSN - 1752-4571
DOI - 10.1111/eva.12301
Subject(s) - biology , dengue fever , aedes aegypti , biological dispersal , genetic variation , aedes , vector (molecular biology) , wolbachia , mitochondrial dna , mosquito control , population , genetic structure , genetics , genetic diversity , evolutionary biology , virology , ecology , gene , malaria , immunology , recombinant dna , demography , sociology , larva
Dengue is the most prevalent global arboviral disease that affects over 300 million people every year. Brazil has the highest number of dengue cases in the world, with the most severe epidemics in the city of Rio de Janeiro (Rio). The effective control of dengue is critically dependent on the knowledge of population genetic structuring in the primary dengue vector, the mosquito Aedes aegypti . We analyzed mitochondrial and nuclear genomewide single nucleotide polymorphism markers generated via Restriction‐site Associated DNA sequencing, as well as traditional microsatellite markers in Ae. aegypti from Rio. We found four divergent mitochondrial lineages and a strong spatial structuring of mitochondrial variation, in contrast to the overall nuclear homogeneity across Rio. Despite a low overall differentiation in the nuclear genome, we detected strong spatial structure for variation in over 20 genes that have a significantly altered expression in response to insecticides, xenobiotics, and pathogens, including the novel biocontrol agent Wolbachia . Our results indicate that high genetic diversity, spatially unconstrained admixing likely mediated by male dispersal, along with locally heterogeneous genetic variation that could affect insecticide resistance and mosquito vectorial capacity, set limits to the effectiveness of measures to control dengue fever in Rio.