Open AccessInvestigating the genetics of B ti resistance using m RNA tag sequencing: application on laboratory strains and natural populations of the dengue vector A edes aegypti
Author(s) -
Paris Margot,
Marcombe Sebastien,
Coissac Eric,
Corbel Vincent,
David JeanPhilippe,
Després Laurence
Publication year - 2013
Publication title -
evolutionary applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.776
H-Index - 68
ISSN - 1752-4571
DOI - 10.1111/eva.12082
Subject(s) - biology , genetics , single nucleotide polymorphism , gene , aedes aegypti , bacillus thuringiensis , genotype , bacteria , botany , larva
Abstract Mosquito control is often the main method used to reduce mosquito‐transmitted diseases. In order to investigate the genetic basis of resistance to the bio‐insecticide B acillus thuringiensis subsp. israelensis ( B ti ), we used information on polymorphism obtained from c DNA tag sequences from pooled larvae of laboratory B ti ‐resistant and susceptible A edes aegypti mosquito strains to identify and analyse 1520 single nucleotide polymorphisms ( SNP s). Of the 372 SNP s tested, 99.2% were validated using DNA I llumina G olden G ate ® array, with a strong correlation between the allelic frequencies inferred from the pooled and individual data ( r = 0.85). A total of 11 genomic regions and five candidate genes were detected using a genome scan approach. One of these candidate genes showed significant departures from neutrality in the resistant strain at sequence level. Six natural populations from M artinique I sland were sequenced for the 372 tested SNP s with a high transferability (87%), and association mapping analyses detected 14 loci associated with B ti resistance, including one located in a putative receptor for C ry11 toxins. Three of these loci were also significantly differentiated between the laboratory strains, suggesting that most of the genes associated with resistance might differ between the two environments. It also suggests that common selected regions might harbour key genes for B ti resistance.