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Test performance study of isothermal amplification tests for the detection of Grapevine flavescence dorée phytoplasma and ‘ Candidatus Phytoplasma solani’
Author(s) -
Mehle N.,
Kogovšek P.,
Constable F.,
De Jonghe K.,
Loiseau M.,
Veratti F.,
Marzachi C.,
Ferretti L.,
Angelini E.,
Filippin L.,
Sousa E.,
Andrade E.,
Hodgetts J.,
Dickinson M.,
Dermastia M.
Publication year - 2017
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/epp.12351
Subject(s) - phytoplasma , loop mediated isothermal amplification , biology , horticulture , polymerase chain reaction , dna , restriction fragment length polymorphism , genetics , gene
This test performance study ( TPS ) was carried out on DNA samples from grapevine, clematis, fungi and bacteria to compare and validate loop‐mediated isothermal amplification (LAMP) tests for detection of Grapevine flavescence dorée phytoplasma and ‘ Candidatus Phytoplasma solani’ (Grapevine Bois noir phytoplasma). Two LAMP tests, for Grapevine flavescence dorée phytoplasma and ‘ Candidatus Phytoplasma solani’ (as developed by Kogovšek and colleagues), with proven applicability for rapid laboratory or on‐site detection were included in this study. They were performed in 10 laboratories. In addition, the commercial Qualiplante/Hyris isothermal amplification test for Grapevine flavescence dorée phytoplasma was performed in three laboratories. The accuracy of the three tests was shown to be over 98%. Moreover, the high accuracy of these tests, which used different devices across different laboratories, confirmed their reproducibility.