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Incidence and spread of knockdown resistance ( kdr ) in G erman C olorado potato beetle ( L eptinotarsa decemlineata S ay) populations
Author(s) -
Tebbe C.,
Breckheimer B.,
Racca P.,
Schorn C.,
Kleinhenz B.,
Nauen R.
Publication year - 2016
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/epp.12265
Subject(s) - leptinotarsa , colorado potato beetle , knockdown resistance , sodium channel , biology , gene knockdown , pest analysis , toxicology , sodium , horticulture , chemistry , pesticide , genetics , ecology , deltamethrin , gene , cyfluthrin , organic chemistry
The C olorado beetle, L eptinotarsa decemlineata ( S ay), is one of the most important pests in many potato‐producing regions. Colorado beetle infestations are normally kept under economic damage thresholds by applying insecticides such as pyrethroids. Pyrethroids are known to act on voltage‐gated sodium channels and have been used for several decades to control L . decemlineata . Their continuous and widespread use has resulted in the development of resistance, which is often linked to a L 1014 F target‐site mutation in the voltage‐gated sodium channel, known as knockdown resistance ( kdr ). Since pyrethroids are used in many potato‐growing regions in G ermany, more than 140 L . decemlineata samples were collected and analysed for the presence of the kdr allele by pyrosequencing diagnostics. Results showed that kdr is present in many G erman L . decemlineata populations, but even its homozygous presence does not substantially compromise the efficacy of recommended label rates of pyrethroids, as demonstrated by bioassays and crossing experiments. The implications of these findings for resistance management are briefly discussed.