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Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK 1‐ WG 4 report of the AES / ILAE Translational Task Force of the ILAE
Author(s) -
Raimondo Joseph V.,
Heinemann Uwe,
Curtis Marco,
Goodkin Howard P.,
Dulla Chris G.,
Janigro Damir,
Ikeda Akio,
Lin ChouChing K.,
Jiruska Premysl,
Galanopoulou Aristea S.,
Bernard Christophe
Publication year - 2017
Publication title -
epilepsia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.687
H-Index - 191
eISSN - 1528-1167
pISSN - 0013-9580
DOI - 10.1111/epi.13901
Subject(s) - epileptogenesis , epilepsy , neuroscience , task (project management) , psychology , medicine , pharmacology , engineering , systems engineering
Summary In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.