Premium
Hippocampal expression of heat shock proteins in mesial temporal lobe epilepsy with psychiatric comorbidities and their relation to seizure outcome
Author(s) -
Kandratavicius Ludmyla,
Hallak Jaime E.,
Carlotti Carlos G.,
Assirati Joao A.,
Leite Joao P.
Publication year - 2014
Publication title -
epilepsia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.687
H-Index - 191
eISSN - 1528-1167
pISSN - 0013-9580
DOI - 10.1111/epi.12787
Subject(s) - subiculum , hippocampal formation , epilepsy , psychosis , hippocampus , heat shock protein , temporal lobe , hippocampal sclerosis , epilepsy surgery , epileptogenesis , psychology , hsp70 , neuroscience , medicine , psychiatry , biology , dentate gyrus , genetics , gene
Summary Objective Biologic substrates behind the close association between mesial temporal lobe epilepsy ( MTLE ) and psychiatric comorbidities are largely unknown. Heat shock protein 70 ( HSP 70) and HSP 90 are ubiquitous molecular chaperones that play important roles in functions from cellular stress response to receptor trafficking control. There are controversial findings regarding HSP expression in epilepsy. Our goal was to examine HSP 70 and HSP 90 expression within the human hippocampal formation of MTLE patients with and without comorbid major depression and psychosis. In addition, we investigated the possible correlation between HSP expression and seizure outcome. Methods MTLE hippocampi of subjects without psychiatric history, MTLE and major depression, and MTLE and interictal psychosis derived from epilepsy surgery and control necropsies were investigated for neuronal densities, HSP 70 and HSP 90 immunoreactive area. Results Increased HSP expression in MTLE and decreased HSP expression in MTLE with psychosis cases were detailed. Patients taking fluoxetine showed increased HSP 90 expression in CA 1, and those taking haloperidol decreased HSP 90 in the granular layer and subiculum. MTLE patients with complete seizure remission presented with decreased HSP 70 expression in CA 4 and subiculum and decreased HSP 90 expression in the granular layer. Significance The present results provide the first demonstration of HSP expression in human MTLE hippocampal formation with and without psychiatric comorbidities. Distinct HSP 70 and HSP 90 expression might explain some of the structural and synaptic alterations differentially regulated in MTLE with and without psychiatric comorbidities. Increased HSP s expression in key hippocampal subfields would reflect increased epileptogenicity and poorer outcome of epilepsy surgery.